Part:BBa_K3812002

Encoding UDP-Glucose-Pyrophosphorylase

BBa_K3812002 can encode UGPase.In higher plants, UGPase is a key enzyme for carbohydrate metabolism, catalyzing the reversible production of glucose-1 phosphate (Glc 1-P) and UTP to udp-glucose and pyrophosphate (PPi). Studies have found that the overexpression of UGPase in hybrid poplars can make carbon flow to the synthesis of starch and cellulose . Silencing UGPase in Phaeodactylum tricornutum can significantly increase the lipid content of Phaeodactylum tricornutum. According to the results observed by LSM, the oil content of Phaeodactylum tricornutum overexpressing UGP was significantly lower than that of the wild type, and the chlorophyll content showed a very significant increase. Therefore, we infer that the UGP overexpression system changed the carbon flow direction of Phaeodactylum tricornutum, from lipid to carbohydrate, and increased the absorption of CO2 by photosynthesis. Eventually lead to an increase in chlorophyll content.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 336
  Illegal EcoRI site found at 1459
  Illegal EcoRI site found at 1879
  Illegal PstI site found at 952
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 336
  Illegal EcoRI site found at 1459
  Illegal EcoRI site found at 1879
  Illegal NheI site found at 613
  Illegal PstI site found at 952
  Illegal NotI site found at 2284
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 336
  Illegal EcoRI site found at 1459
  Illegal EcoRI site found at 1879
  Illegal BamHI site found at 2892
  Illegal XhoI site found at 457
  Illegal XhoI site found at 1299
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 336
  Illegal EcoRI site found at 1459
  Illegal EcoRI site found at 1879
  Illegal PstI site found at 952
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 336
  Illegal EcoRI site found at 1459
  Illegal EcoRI site found at 1879
  Illegal PstI site found at 952
  Illegal NgoMIV site found at 462
  Illegal AgeI site found at 1327
  
• 1000
  COMPATIBLE WITH RFC[1000]